Paul Scherrer Institut
Biomolecular Research
OFLC/111
CH- 5232 Villigen PSI
Switzerland

Phone: +41-(0)56-310-4754
Fax: +41-(0)56-310-5288
E-mail: Michel Steinmetz

PERSONAL INFORMATION:

Nationality: Swiss
Date of Birth: 16.1.1967
Married, three daughters

EDUCATION AND ACADEMIC CAREER

Ph.D. in Biochemistry , M.E. Müller-Institut at the Biozentrum of the University of Basel, Switzerland, 1992-1996 . Summa cum laude graduation.

Post Doctoral Fellow , M.E. Müller-Institut at the Biozentrum of the University of Basel, Switzerland. 1996-1998.

Post Doctoral Fellow , Novartis Pharma AG, Functional Genomics Area, Protein Sciences Unit, Basel, Switzerland. 1998-2000.

Senior Research Scientist , Biomolecular Research, Structural Biology, Paul Scherrer Institut, Villigen PSI, Switzerland. 2000-2005.

Group Leader , Biomolecular Research, Structural Biology, Paul Scherrer Institut, Villigen PSI, Switzerland. 2006-present.

AWARDS:

1999: Amerbach-Prize of the University of Basel, Switzerland

RESEARCH GRANTS:

2001: FNSNF Grant 31-64978.01 (principle investigator)

2001: ETHZ Grant TH-42/02-2 (co-investigator)

2005: FNSNF Grant 3100A0-109423 (principle investigator)

2008: FNSNF Grant 3100A0-122545 (principle investigator)

2009: FNSNF Sinergia Grant CRSII3_125463 (co-investigator)

LIST OF PUBLICATIONS

2010

65. Macdonald, P.R., Lustig, A., Steinmetz, M.O. and Kammerer, R.A.
Laminin chain assembly is regulated by specific coiled-coil interactions.
J. Struct. Biol., in press.

64. Lachkar, S., Lebois, M., Steinmetz, M.O., Guichet, A., Lal, N., Curmi, P.A., Sobel, A. and Ozon, S.
Drosophila stathmins bind tubulin heterodimers with high and variable stoichiometries.
J. Biol. Chem., 285, 11667-11680 (2010).

63. De Groot, C.O., Jelesarov, I., Damberger, F.F., Bjelic, S., Schaerer, M.A., Bhavesh, N.S., Grigoriev, I., Buey, R.M., Wuthrich, K., Capitani, G., Akhmanova, A. and Steinmetz, M.O. Molecular insights into mammalian end binding protein heterodimerization.
J. Biol. Chem. 285, 5802-5814 (2010).

2009

62. Honnappa, S., Montenegro Gouveia, S., Weisbrich, A., Damberger, F.F., Bhavesh, N.S., Jawhari, H., Grigoriev, I., van Rijssel, F.J.A., Buey, R.M., Lawera, A., Jelesarov, I., Winkler, F.K., Wüthrich, K., Akhmanova, A. and Steinmetz, M.O.
An EB1-binding motif acts as a microtubule tip localization signal.
Cell 138, 366-376 (2009).

61. Nie, Y., Bieniossek, C., Frey, D., Olieric, N., Schaffitzel, C., Steinmetz, M.O. and Berger, I.
ACEMBLing multigene expression vectors by recombineering.
Nat. Protoc. doi: 10.1038/nprot.2009.104.

60. Manna, T., Thrower, D.A., Honnappa, S., Steinmetz, M.O. and Wilson, L.
Regulation of Microtubule Dynamic Instability in vitro by Differentially Phosphorylated Stathmin.
J. Biol. Chem. 284, 15640-15649 (2009).

59. Wasmer, C., Benkemoun, L., Sabaté, R., Steinmetz, M.O., Coulary-Salin, B., Wang, L., Riek, R., Saupe, S. and Meier, B.H.
Solid-State NMR reveals that E. coli inclusion bodies of HET-s(218-289) are amyloids.
Angew. Chem. Int. Ed. Engl. 48, 4858-4860 (2009).

58. Bieniossek, C., Nie, Y., Frey, D., Olieric, N., Schaffitzel, C., Collinson, I., Romier, C., Berger, P., Richmond, T.J., Steinmetz, M.O. and Berger, I.
Automated unrestricted multigene recombineering for multiprotein complex production.
Nat. Methods 6, 447-450 (2009).

57. Komarova, Y., De Groot, C.O., Grigoriev, I., Montenegro Gouveia, S., Munteanu, L., Schober, J.M., Honnappa, S., Buey, R.M., Hoogenraad, C.C., Dogterom, M., Borisy, G.G., Steinmetz, M.O. and Akhmanova, A.
Mammalian EB proteins control persistent microtubule growth.
J. Cell Biol., 184, 691-706 (2009).

2008

56. Steinmetz, M.O. and Akhmanova, A.
Capturing protein tails by CAP-Gly domains.
Trends Biochem. Sci. 33, 535 - 545 (2008)

55. Hwang, S.J., Kim, S.H., Kim, H.Z., Steinmetz, M.O., Koh, G.Y. and Lee, G.M.
High-level Expression and Purification of a Designed Angiopoietin-1 Chimeric Protein, COMP-Ang1, Produced in Chinese Hamster Ovary Cells.
Protein J., 27, 319-326 (2008)

54. Dolenc, J., Baron, R., Missimer, J.H., Steinmetz, M.O. and van Gunsteren, W.F.
Exploring the conserved water sites and hydration of a coiled-coil trimerization motif: A MD simulation study.
Chembiochem. 9, 1749 - 1756 (2008)

53. Verel, R., Tomka, I.T., Bertozzi, C., Cadalbert, R., Kammerer, R.A., Steinmetz, M.O. and Meier, B.H.
Polymorphism in an amyloid-like fibril forming model peptide.
Angew. Chem. 47, 5842 - 5845 (2008)

52. Akhmanova, A. and Steinmetz, M.O.
Traking the ends: a dynamic protein network controls the fate of microtubule tips.
Nat. Rev. Mol. Cell Biol. 9, 302 - 322 (2008)

51. Grigoriev, I., Montenegro Gouveia, S., van der Vaart, B., Demmers, J., Smyth, J.T., Honnappa, S., Splinter, D., Steinmetz, M.O., Putney, J.W., Hoogenraad, C.C. and Akhmanova, A.
STIM1 is a MT-plus-end-tracking protein involved in remodeling of the ER. 
Curr. Biol. 18, 177 - 182 (2008)

50. Steinmetz, M.O., Gattin, Z., Verel, R., Ciani, B., Stromer, T., Green, J.M., Tittmann, P., Schulze-Briese, C., Gross, H., van Gunsteren, W.F., Meier, B.H., Serpell, L.C., Müller, S.A. and Kammerer, R.A.
Atomic models of de novo designed ccb-Met amyloid-like fibrils.
J. Mol. Biol. 376, 898 - 912 (2008)

49. Manna, T., Honnappa, S., Steinmetz, M.O., and Wilson, L.
Suppression of Microtubule Dynamic Instability by the +TIP Protein EB1 and its Modulation by the CAP-Gly Domain of p150Glued. 
Biochemistry 47, 779-786 (2008)

2007

48. Teuling, E., Ahmed, S., Haasdijk, E., Demmers, J., Steinmetz, M.O., Akhmanova, A., Jaarsma, D. and Hoogenraad, C.C.
Motor neuron disease-associated mutant VAPB recruits wild type VAPs into ER-derived tubular aggregates.
J. Neursci., 27, 9801 - 9815 (2007).

47. Weisbrich, A., Honnappa, S., Jaussi, R., Okhrimenko, O., Frey, D., Jelesarov, I., Akhmanova, A., and Steinmetz, M.O.
Structure-function relationship of CAP-Gly domains.
Nat. Struct. Mol. Biol., 14, 959 - 967 (2007).

46. Scherr, N., Honnappa, S., Kunz, G., Mueller, P., Jayachandran, R., Winkler, F.K., Pieters, J., and Steinmetz, M.O.
Structural Basis for the specific inhibition of protein kinase G, a virulence factor of Mycobacterium tuberculosis.
Proc. Natl. Acad. Sci. USA, 104, 12151 - 12156 (2007).

45. Frey, D., Kambach, C., Steinmetz, M.O. and Jaussi, R.
Production of in vitro amplified DNA pseudolibraries and high-throughput cDNA target amplification.
BMC Biotechnology, 7, 31 (2007).

44. John, C.M., Hite, R.K., Weirich, C.S., Fitzgerald, D.J., Faty, M., Jawhari, H., Schlaepfer, D., Kroschewski, R., Winkler, F.K., Barral, Y., Steinmetz, M.O.
The Caenorhabditis elegans septin complex is non-polar.
EMBO J., 26, 3296 - 3307 (2007)

43. Missimer, J.H., Steinmetz, M.O., Baron, R., Winkler, F.K., Kammerer, R.A., Daura, X., and van Gunsteren, W.F.
Configurational entropy elucidates the role of salt-bridge networks in protein thermostability.
Prot. Sci., 16, 1349 - 1359 (2007)

42. Steinmetz, M.O., Jelesarov, I., Matousek, W.M., Honnappa, S., Jahnke, W., Missimer, J.H., Frank, S., Alexandrescu, A.T. and Kammerer, R.A.
Molecular basis of coiled-coil formation.
Proc. Natl. Acad. Sci. USA , 104 , 7062-7067 (2007)

41. Ruch, C., Skiniotis, G., Steinmetz, M.O., Walz, T., and Ballmer-Hofer, K.
Structure of a VEGF / VEGF-receptor complex as determined by EM.
Nat. Struct. Mol. Biol.14, 249-250 (2007)

40. Steinmetz, M.O. Structure and thermodynamics of the tubulin-stathmin interaction. J. Struct. Biol.158, 137 - 147 (2007)

2006

39. Macdonald, P.R., Progias, P., Ciani, B., Patel, S., Mayer, U., Steinmetz, M.O., and Kammerer, R.A.
Structure of the extracellular domain of Tie receptor tyrosine kinases and localization of the Angiopoietin-binding epitope.
J. Biol. Chem. 281:28408-28414 (2006)

38. Honnappa, S., Okhrimenko, O., Jaussi, R., Jawhari, H., Jelesarov, I., Winkler, F.K., and Steinmetz, M.O.
Key interaction modes of dynamic +TIP networks.
Mol. Cell 23 , 663-671 (2006)

37. Honnappa, S., W. Jahnke, J. Seelig and M.O. Steinmetz.
Control of intrinsically disordered stathmin function by multisite phosphorylation.
J. Biol. Chem. 281, 16078-16083 (2006).

36. Kammerer, R.A. and Steinmetz, M.O.
De novo design of a two-stranded coiled-coil switch peptide.
J. Struct. Biol. 155, 146-153 (2006)

35. Siemer, A.B., Ritter, C., Steinmetz, M.O., Ernst, M., Riek, R. and Meier, B.H.
13C, 15N resonance assignment of parts of the HET-s prion protein in its amyloid form.
J. Biomol. NMR 34, 75-87 (2006).

2005

34. Kammerer, R.A., Kostrewa, Progias, P., Honnappa, S., D., Avilla, D., Lustig A., Winkler, F.K., Pieters, J. and Steinmetz, M.O.
A conserved trimerization motif controls the topology of short coiled coils.
Proc. Natl. Acad. Sci. USA 102, 13891-13896 (2005).

33. Missimer, J.H., Steinmetz, M.O., Jahnke, W. Winkler, F.K., van Gunsteren, W.F., and Daura, X.
Molecular dynamics simulations of helical 16-residue C- and N-terminal peptide derivatives of GCN4-p1 in aqueous solution.
Chem. Biodiv. 2, 1086-1104 (2005).

32. Gigant, B., Wang, C., Ravelli, R., Roussi, F., Steinmetz, M.O., Curmi, P., Sobel, A. and Knossow, M.
Structural insights into tubulin regulation by vinblastine.
Nature 435, 519-522 (2005).

31. Gatfield, J., Zanalori, B., Steinmetz, M.O. and Pieters, J.
Association of the leukocyte plasma membrane with the actin cytoskeleton through coiled-coil-mediated trimeric coronin 1 molecules.
Mol. Biol. Cell 16, 2786-2798 (2005).

30. Kim, K.T., Choi, H.H., Steinmetz, M.O., Maco, B., Kammerer, R.A., Ahn, S.Y., Kim, H.K., Lee, G.M. and Koh, G.Y.
Oligomerization and multimerization is critical for angiopoietin-1 to bind and phosphorylate tie2.
J. Biol. Chem. 280, 20126-20131 (2005).

29. Steinmetz, M.O., García-Echeverría, C. and Kammerer, R.A.
Design of a coiled-coil-based model peptide system to explore the fundamentals of amyloid formation.
Int. J. Pep. Res. Ther. 11, 43-52 (2005).

28. Honnappa, S., John, C.M., Kostrewa, D., Winkler, F.K. and Steinmetz M.O.
Structural insights into the EB1-APC interaction.
EMBO J. 24, 261-269 (2005).

2004

27. Cho C.H., Kammerer, R.A., Lee, H.J., Steinmetz, M.O., Ryu, Y.S., Lee, S.H., Yasunaga, K., Kim, K.T., Kim, I., Choi, H.H., Kim, W., Kim, S.H., Park, S.K., Lee, G.M. and Koh, G.Y.
COMP-Ang1: a designed angiopoietin-1 variant with nonleaky angiogenic activity.
Proc. Natl. Acad. Sci. USA 101, 5547-5552 (2004).

26. Kammerer, R.A., Kostrewa, D., Zurdo, J., Detken, A., García-Echeverría, C., Green, J.D., Müller, S.A., Meier, B.H., Winkler, F.K., Dobson, C.M. and Steinmetz, M.O.
Exploring amyloid formation by a de novo design.
Proc. Natl. Acad. Sci. USA 101, 4435-4440 (2004).

2003

25. Honnappa, S., Cutting, B., Jahnke, W., Seelig, J. and Steinmetz M.O.
Thermodynamics of the Op18/stathmin-tubulin interaction.
J. Biol. Chem. 278, 38926-38934 (2003).

2001

24. Steinmetz, M.O. , Jahnke, W., Towbin, H., Garc ía-Echeverr ía, C., Voshol, H., Müller, D. and van Oostrum, J.
Phosphorylation disrupts the central helix in Op18/stathmin and suppresses binding to tubulin.
EMBO Rep. 2, 505-510 (2001).

23. Müller, D.R., P. Schindler, H. Towbin, U. Wirth, H. Voshol, S. Hoving and M.O. Steinmetz.
Isotope-tagged cross-linking reagents. A new tool in mass spectrometric protein interaction analysis.
Anal. Chem. 27, 1927-1934 (2001).

22. Kammerer, R.A., V.A. Jaravine, S. Frank, T. Schulthess, R. Landwehr, A. Lustig, C. García-Echeverría, A.T. Alexandrescu, J. Engel and M.O. Steinmetz.
An intrahelical salt bridge within the trigger site stabilizes the GCN4 leucine zipper.
J. Biol Chem. 276, 13685-13688 (2001).

2000

21. Wohlsland, F., M.O. Steinmetz, U. Aebi and G. Vergères.
MARCKS-related protein (MRP) binds to actin without significantly affecting actin polymerization or network structure.
J. Struct. Biol. 131, 217-224 (2000).

20. Steinmetz, M.O. , A. Hoenger, D. Stoffler, A.A. Noegel, U. Aebi and C.-A. Schoenenberger.
Polymerization, Three-dimensional structure and mechanical properties of dictyostelium versus rabbit muscle actin filaments.
J. Mol. Biol. 303, 171-184 (2000).

19. Wohnsland, F., A.A.P. Schmitz, M.O. Steinmetz, U. Aebi and G. Vergères.
Influence of the effector peptide of MARCKS-related protein on actin polymerization: a kinetic analysis.
Biophys. Chem . 85, 169-177 (2000).

18. Wohlsland, F., A.A.P. Schmitz, M.O. Steinmetz, U. Aebi and G. Vergères.
Interaction between actin and the effector peptide of MARCKS-related protein (MRP): Identification of functional amino acid segments.
J. Biol. Chem. 275, 20873-20879 (2000).

17. Burkhard, P., R.A. Kammerer, M.O. Steinmetz , Bourenkov, G.P. and U. Aebi.
The coiled-coil trigger site of the rod domain of cortexillin I unveils a distinct network of interhelical and intrahelical salt bridges.
Structure 8, 223-230 (2000).

16. Steinmetz, M.O., R.A. Kammerer, W. Jahnke, K.N. Goldie, A. Lustig and J. van Oostrum.
Stathmin/Op18 caps a kinked protofilament-like tubulin tetramer.
EMBO J., 19, 572-580 (2000).

15. De La Cruz, E.M., A. Mandinova, M.O. Steinmetz, D. Stoffler, U. Aebi and T.D. Pollard.
Nucleotide dependence of actin polymerization, structure and dynamics.
J. Mol. Biol., 295, 517-526 (2000).

1999

14. Stoffler, D. M.O. Steinmetz and U. Aebi.
Imaging biological matter across dimensions: from cells to molecules and atoms.
FASEB J., 13, S195-S200 (1999).

13. Stock, A., M.O. Steinmetz, P.A. Janmey, U. Aebi, G. Gerisch, R.A. Kammerer, I. Wagner and J. Faix.
Domain analysis of cortexillin I: actin-bundling and PIP2-binding in relation to the rescue of cytokinesis.
EMBO J . 18, 5274-5284 (1999).

12. Schoenenberger, C.-A., M.O. Steinmetz, D. Stoffler, A. Mandinova and U. Aebi.
Structure, assembly, and dynamics of actin filaments in situ and in vitro.
Microsc. Res. Tech. 47, 38-50 (1999).

11. Spiess, M., M.O. Steinmetz, A. Mandinova, B. Wolpensinger, U. Aebi and D. Atar.
Isolation, electron microscopic imaging and 3-D visualization of native cardiac thin myofilaments.
J. Struct. Biol. 126, 98-104 (1999).

1998

10. Kammerer, R.A., T. Schulthess, R. Landwehr, A. Lustig, J. Engel, U. Aebi and M.O. Steinmetz.
An autonomous folding unit mediates the assembly of two-stranded coiled coils.
Proc. Natl. Acad. Sci. USA 95, 13419-13424 (1998).

9. Steinmetz, M.O., C. Plüss, U. Christen, B. Wolpensinger, A. Lustig, E.R. Werner, H. Wachter, A. Engel, U. Aebi, J. Pfeilschifter and R.A. Kammerer.
Rat GTP cyclohydrolase I is a homodecameric protein complex containing high-affinity calcium-binding sites.
J. Mol. Biol. 279, 189-199 (1998).

8. Burkhard, P., M.O. Steinmetz, T. Schulthess, R. Landwehr, U. Aebi and R.A. Kammerer.
Crystallization and preliminary X-ray diffraction analysis of the 190 Å long coiled-coil dimerization domain of the actin-bundling protein cortexillin I from Dictyostelium discoideum.
J. Struct. Biol. 122, 293-296 (1998).

7. Steinmetz, M.O., A. Hoenger, P. Tittmann, K.H. Fuchs, H. Gross and U. Aebi.
An atomic model of crystalline actin tubes: combining electron microscopy with X-ray crystallography.
J. Mol. Biol. 278, 703-711 (1998).

6. Steinmetz, M.O., D. Stoffler and U. Aebi.
Actin: Dissecting the structural basis of its oligomerization, Polymerization, and polymorphism.
Biol. Bull. 194, 337-341 (1998).

5. Steinmetz, M.O., D. Stoffler, S. Müller, W. Jahn, B. Wolpensinger, K.N. Goldie, A. Engel, H. Faulstich and U. Aebi.
Evaluating atomic models of F-actin with and undecagold-tagged phalloidin derivative.
J. Mol. Biol. 276, 1-6 (1998).

4. Steinmetz, M.O., A. Stock, T. Schulthess, R. Landwehr, A. Lustig, J. Faix, G. Gerisch, U. Aebi and R.A. Kammerer.
A distinct 14-residue site triggers coiled-coil formation in cortexillin I.
EMBO J. 17, 1883-1891 (1998).

1997

3. Steinmetz, M.O., K.N. Goldie and U. Aebi.
A correlative analysis of actin filament assembly, structure and dynamics
J. Cell Biol. 138, 559-574 (1997).

2. Steinmetz, M.O., D. Stoffler, A. Bremer, A. Hönger and U. Aebi.
Actin: from cell biology to atomic details.
J. Struct. Biol. 119, 295-320 (1997).

1996

1. Faix, J., M.O. Steinmetz, H. Boves, R.A. Kammerer, F. Lottspeich, U. Minert, J. Murphy, A. Stock, U. Aebi and G. Gerisch.
Cortexillins, major determinants of cell shape and size, are actin-bundling proteins with a parallel coiled-coil tail.
Cell 86, 631-642 (1996).

Contact Address:

Michel O. Steinmetz
Paul Scherrer Institut
OFLC/111
CH-5232 Villigen PSI
Switzerland

Phone:   +41-56-310-4754
Fax:     +41-56-310-5288
E-mail: michel.steinmetz@psi.ch

Secretary:
Monika Brunner
Phone: +41-56-310-4252
Fax:     +41-56-310-5288
E-mail: monika.brunner@psi.ch